A study published in the journal Pharmacy has validated a new method for accurately quantifying cannabinoids in commercial veterinary CBD oil.
(Photo credit: Healthline).
According to its abstract, the study “highlights the need for quality control in evaluating medicinal plant products, especially CBD oils, before market release”, noting that “Due to varying regulatory requirements, product labeling can sometimes be misleading, especially regarding cannabinoid concentrations such as CBD and THC.”
With that in mind, this research “focused on developing a validated high-performance liquid chromatography (HPLC) method for accurately identifying and quantifying key cannabinoids in Commercial Veterinary CBD Oil.”
The main compounds identified included Cannabidivarin (CBDV), Cannabidiolic Acid (CBD-A), Cannabigerolic Acid (CBG-A), Cannabigerol (CBG), Cannabidiol (CBD), Tetrahydrocannabivarin (THCV), Cannabinol (CBN), ∆9-Tetrahydrocannabinol (d9-THC) ∆8-Tetrahydrocannabinol (d8-THC), Cannabicyclol (CBL), Cannabichromene (CBC), and Tetrahydrocannabinolic Acid (THCA), determined in line with the International Conference on Harmonization’s (ICH) guidelines.
The method underwent rigorous validation for linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ), meeting high analytical standards. Researchers found the method to be linear, with a correlation coefficient (R²) exceeding 0.999. Detection and quantification limits ranged from 0.05 to 0.13 µg/mL and 0.50 to 0.61 µg/mL, respectively. Precision was demonstrated with relative standard deviation values at or below 2%, and accuracy was confirmed through recovery percentages within an impressive range of 98% to 102%.
In their analysis of 14 commercial veterinary CBD oil products from North Macedonia, researchers applied this validated protocol to quantify 12 cannabinoids.
The study reported that cannabinoid levels in the oils largely aligned with the manufacturers’ labeling claims.
“The analysis showed that the cannabinoid levels in the products were consistent with the manufacturers’ declared specifications, with no significant discrepancies in labeling”, concludes the study, the full text of which can be found by clicking here.
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